Journal: Frontiers in Immunology
Article Title: Effect of seasonal coronavirus immune imprinting on the immunogenicity of inactivated COVID-19 vaccination
doi: 10.3389/fimmu.2023.1195533
Figure Lengend Snippet: Seroprevalence of sCoVs and SARS-CoV-2 S IgG antibodies in different cohorts.
Article Snippet: Briefly, to detect corresponding antibodies, 96-well EIA/RIA plates (Corning, #3590) were coated with recombinant SARS-CoV-2 full-length S (Sino Biological, #40591-V08B1-1), SARS-CoV-2 S1(Sino Biological, #40591-V08H), S2 (Sino Biological, #40590-V08B), SARS-CoV-2 full-length N (Sino Biological, #40588-V08B), HCoV-HKU1 full-length S (Sino Biological, #40606-V08B), HCoV-NL63 full-length S (Sino Biological, #40604-V08B), HCoV-OC43 full-length S (Sino Biological, #40607-V08B), and HCoV-229E full-length S proteins (Sino Biological, #40605-V08B) at 100ng/ul in ELISA coating buffer (Solarbio, #C1055) respectively, and incubated at 4°C overnight.
Techniques: